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TABLE 16-1 Uptake-Targeting Sequences That Direct Proteins from the Cytosol to Organelles Target Organelle Endoplasmic reticulum (lumen) Mitochondrion (matrix) Chloroplast (stroma) Peroxisome (matrix) Location of Sequence Within Protein N-terminus N-terminus N-terminus C-terminus (most proteins); N-terminus (few proteins) Removal of Sequence Nature of Sequence Yes Core of 612 hydrophobic amino acids, often preceded by one or more basic amino acids (Arg, Lys) Yes Amphipathic helix, 2050 residues in length, with Arg and Lys residues on one side and hydrophobic residues on the other Yes No common motifs; generally rich in Ser, Thr, and small hydrophobic residues and poor in Glu and Asp No PTS1 signal (Ser-Lys-Leu) at extreme C-terminus; PTS2 signal at N-terminus Different or additional sequences target proteins to organelle membranes and subcompartments.

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